You cannot use the PCI-GPIB board in the DOS operating system. You can however, you can use DOS programs through the Windows DOS prompt if you enable DOS support for the NI-488.2 driver.
http://ae.natinst.com/operations/ae/public.nsf/fca7838c4500dc10862567a100753500/5d73dffd66f4ebed8525639a00455379?OpenDocument
http://ae.natinst.com/operations/ae/public.nsf/fca7838c4500dc10862567a100753500/862567530005f09c862565850060ce6d?OpenDocument
Kim L
Applications Engineer