Agaa,
When you do an AI Read, the data must be available to read from the buffer, or it waits until it is there. Therefore, the Data Acquisition thread is locked up, and no other data acquisition VIs will run until this AI Read is finished.
To get around this problem, check out the example /examples/daq/anlogin/anlogin.llb/Cont Acq&Chart (Async Occurrence).vi. This VI uses occurrences (like an interrupt), where the AI Read waits until the occurrence is set, and the occurrence is set when the data is available in the buffer. This allows your CPU to do other functions in the meantime, including other data acquisition calls.
Mark