Hello,
I don't know if this is the same issue as the one in another Discussion Forum about reading a spreadsheet and passing the values from the host computer to the RT box.
http://forums.ni.com/ni/board/message?board.id=170&message.id=110228
If it is, I just posted a new response about the how to use VI server and RT communication wizard.
If you try to use your AO,and those AO are in the remote RT system, then you cannot run this VI in Windows. You would need to run it in the RT. You will probably need to change the VI so that the read from spreadsheet is done in the host computer while writing to the AO is done in the RT.
Hope this helps.
Ricardo S.
National Instruments