Thanks for the suggestion Altenbach. I had to insert a for loop to replace array subset indivually for each column of new data to place in the buffer, but even with the extra processing, the result was about 80% faster. I guess I'll have to come up with a system to track where to place the data (it was convenient to always drop the data at the end..), but that shouldn't be an issue.
However, this suggestion doesn't address the original concern. The same array resizing was going on in both the subvi and the sequence frame. This improvement should increase the performance of each, but doesn't explain the difference.
As an aside, I contemplated using a queue, but without the ability to dequeue from both the beginning and the end, I don't see how I can use them for this. I want to trash the oldest data, not hold up the acquisition. In any case, I don't think it would be faster because the memory isn't pre-allocated. Now I am considering just storing the data in a control, and passing the reference as necessary. The question is, is reading the value from a control faster than reading from a shift register?
Thanks again.
Chris