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Error -1074396113 Convert Pixel To Real World During Camera Feed

Hello,
 
I am fairly new to LabView and am encountering an error that I can't seem to solve.
 
I am utilizing an image feed from a firewire camera to detect the real world location of a white line.  I have modified a tested, existing VI that processes the stream into individual image files, which I then pass to the VIs I wrote.
 
Using the NI Vision Assistant, I created a script that extracts a color plane and processes the image to a binary image, where "off" is blank space and "on" is the line I am trying to find.  I then pass this image onto another VI, where I am encountering the error "Error -1074396113 occurred at IMAQ Convert Pixel To Real World.  The source/input image has not been calibrated" when I attempt to convert the binary image into real world coordinates using the "convert pixel to real world" VI. 
 
I have calibrated my camera per instructions in the NI Vision Assistant.  Strangely, once the program encounters the error and I stop execution of the VI, then re-run just the image processing VIs on the static image still retained in the wire, everything executes properly.
 
I would appreciate any help in this matter,
 
Thank you,
German Coast Guard
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Hey German Coast Guard,

I like your user name! But that’s beside the point. Please refer to your post in the Machine Vision forums for further discussion of this issue, and to check out my reply. Thanks!

David G
Sales Engineer - SE Michigan & N Ohio
National Instruments
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