Hi Maria,
The error that you are getting is a timeout error which means that samples are not being acquired. The DAQmx read VI is probably in the Read Counters for Initialize.vi. I was wondering if you were using a sample clock or a trigger in this acquisition. For the sample clock, if it is too slow it will cause this error. If it is waiting for a trigger and does not receive one, it will also throw this error. For more information you can check out
this article. If this does not help, please attach the subVIs so we can see where the acquisition actually takes place. I hope this helps.
Regards,
Kent
Applications Engineer