The first thing you need to do is to save top level VI and all the hierarchy into LLB. Then when you select the app builder, just select the top level from the LLB file and build the application.
Second thing is that you need to transport some DLL and files from developement machine to the target machine. you can find a list of those on
http://digital.ni.com/public.nsf/3efedde4322fef19862567740067f3cc/b814be005f9da9258625658700550c75?OpenDocument
Don't forget to create DSN in ODBC
Hope this helps
A Rafiq