You're probably looking for the "Run when Opened" setting. In the build specification, it's under Source File Settings. From there, select your startup VI, then click "Customize VI Properties..." However, if you want a more professional-looking application, put a start command (button, menu, whatever) in your application and wait until that is triggered before using the data, instead of expecting the user to know to click LabVIEW's run button.
