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IMAQ x step y step calibration

I am using LabVIEW and IMAQ and want to calibrate a distance in pixel (I don't have coordinates that make the distance). I looked at the calibration VIs, but there isn't any VI that allow me to convert distance in pixel to real world measurement. So I want to calculate the distance in real world units by using the distance in pixel. Is there anyway to calculate the distance in real world measurement unit if I know how many pixel in X and Y axis (my X Step is not equal to Y Step) is equal to the unit in real world?
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This sounds like the same questions answered on another discussion forum. Take a look at the below link.

http://exchange.ni.com/servlet/ProcessRequest?RHIVEID=101&RPAGEID=135&HOID=50650000000800000091A10000&USEARCHCONTEXT_CATEGORY_0=_15_&USEARCHCONTEXT_CATEGORY_S=0&UCATEGORY_0=_15_&UCATEGORY_S=0
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