Hi,
You should not have too much trouble to the extent of having two programs running at the same time. Deep down in the LV Vi's they call into the dll just like your VB app does. The main problem will be the instrument. If you have two programs accessing the same instrument, you could have trouble if one program expects the instrument to be in a certain state and its not, or they both try to read at the same time, etc, etc, etc.
Hope this helps out!
Best Regards,
Aaron K.
Application Engineer
National Instruments