The path of the directory in which a VI is stored is different before and after building an application.
The VI Path before is Directory\VI, whereas after it's Directory\Application.exe\VI.
Make sure you're stripping the current VIs directory correctly and that as a result, the name of the VI you're trying to load is correct.
Hope this helps
Shane.
Using LV 6.1 and 8.2.1 on W2k (SP4) and WXP (SP2)