Using an image to calibrate a microscope stage using labview

[HumanCondition]

So I'm trying to understand how to use my image as a calibration tool for the NI examples such as nonlinear, perspective, and simple. I was thinking it would be a quick chug and plug with me just putting an image I want in, but that method has failed. I was wondering if anyone could shed some light on this matter and give some tips on how to write this program?

Thanks,

HumanCondition

[smithd]

Hey HumanCondition,

You are correct, it is not quite as simple as that. First, you will need to teach labview the calibration. To do this, you would need a calibration template with a known distance and angle between points. For example, an array of dots. You would need to snap an image of this at the perspective from which you will be taking your images, and then use the distortion to correct future images from the same angle with the same camera. The simple calibration example uses a fixed template from a known angle to perform this same task.

This document:

http://www.ni.com/pdf/manuals/372916g.pdf

Will be your best friend during this process, specifically 3-6 (pdf page 48)

Let me know what questions you have after taking a look through that section.

Thanks,