It sounds like you are publishing everything correctly if it works on one PC but not the other. It could be an issue with the one PC's antivirus software - I have to disable certain settings in my antivirus (Sophos) to get my (LabVIEW Remote Front Panel) webpages to load correctly in the Internet Exploder. Might be worth trying to disable your antivirus temporarily to see if the page starts working. If so, back track from there and see what specific setting is causing it to break.
The other thing to check is that installing the LabVIEW RTE did correctly install the Internet Explorer add-on. Open IE > click gear icon > "Manage Add-ons" > In the "Show:" selection box, choose, "All add-ons". You should see an add-on called, "LabVIEWControl Class" published by National Instruments corporation and it should be enabled (if not enabled, right click and choose, "enable"). If you do not see it, try to re-install the RTE. Depending on the RTE version, there may be a separate browser plug-in that you install (I think LabVIEW 2016 had a separate plug-in, for example).
On a separate note, FYI - Internet Explorer goes out of support sometime in 2022 last I checked, and the Silverlight technology required for browser Remote front Panels (RFPs) went out of support last month. You may want to look into a replacement for your current RFP. I recommend using NI's new G Web Software to create a new webpage which requires no special RTE or plug-in and can run in Edge or Firefox, for example, with no issues.
