1. What's connected to the DAQ? Are you just measuring the voltage off of a breakout box or is it wired into something. If you put too high a load on the board, that can yank it down. These cards only put out a few mA.
2. In MAX (Measurement & Automation Explorer), run the test panels for the DIOs and measure the voltage output to determine if it's software or other.
3. Use the online diagnostic to check your board:
http://www.ni.com/support/selftest/default.htm
or download a diagnostic:
http://sine.ni.com/apps/we/niepd_web_display.display_epd4?p_guid=B45EACE3DCD356A4E034080020E74861&p_node=DZ52334&p_source=external
4. If the above fail, post your vi and we'll see what's up.
2006 Ultimate LabVIEW G-eek.