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differential the edge detected on each of 2 virtual lines

Hello wuliao23,

The IMAQ Edge Tool 2 VI works with a single ROI.  You can learn more about ROIs in the NI Vision for LabVIEW Help, which typically installs as IMAQVision.chm to the following directory:
C:\Program Files\National Instruments\Vision\Documentation

Within the NI Vision for LabVIEW Help, refer to the Define Regions of Interest section, which is nested as follows:
NI Vision for LabVIEW Help>>NI Vision for LabVIEW Basics>>Making Grayscale and Color Measurements>>Define Regions of Interest

As you will see, you can include multiple contours within a single region of interest.

Regarding your second question, you should expect some variation in execution time.  Windows is not a deterministic operating system.  To reduce variation, I suggest you close other programs that may be running in the background.

Best Regards,
T. McCarty
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