What do the 2 columns represent? Is this one or more analog waveforms that you want to output from the DAQ board? Is there timing information included?
Reading a text file is easy with Read From Spreadsheet File and there are a lot of shipping examples for analog output. Basically, you would create a 2D array and wire that to the scaled data input of AO Write so if you could provide details on how the data in the file is stored, it would help.