Nate,
The 6533 is can only be set to 8-bit, 16-bit, or 32-bit when using pattern generation. So in this case you will have to configure at least one 8-bit port for data transfer. But you can simply write the same information out on all the other lines and change only the line of interest.
If you do not want to use pattern I/O which give you hardware timing output then you can simply use the Write to Digital line VI which allows you to update a single line at a time, but the timing of the update is related to software and is indeterministic and relies on the speed of the program execution.
By the way, it is better to post Hardware Data Acquistion questions to one of the Measurement Devices catagories instead of LabVIEW. For example, this question will be seen b
y more people interest in programming Digtial I/O if it is posted under the Digital I/O catagory