Hi,
Why don't you give us a bit more info on your problem; wee may be ablo to help you out.
There is no "known" reason for a NT executable not to work in Windows 2000; however those are 2 different OSes and you may find that some things behave differently.
You may want to upgrade the CVI run-time engine in the targe machine, this may fix some issues that were present in older versions of CVI.
Regards,
Juan Carlos
N.I.