Robert,
The simplest way I can come up with is to use Binary Morphology. If you use Dilate, it will make all your particles grow. By selecting the proper parameters, you should be able to get all your particles to increase their size by half the minimum spacing. If the spacing between two particles was less than the minimum, they should run together and become a single particle. Use particle detection and see if the total number of particles has decreased, and that will tell you if two particles ran together.
If necessary, you could design more elaborate detection algorithms at the end. You could subtract the original image from the dilated image, which would leave holes where the original particles were. Using particle detection, you could count
the number of holes in each particle. I think the result would be the same, though.
You would have to make sure you remove any particles that aren't clusters before doing the processing. Two tiny specs could run together and change your count, when they are just noise.
Bruce
Bruce Ammons
Ammons Engineering