Under binary image analysis in Vision Builder, select particle analysis. The property you want is max intercept. This gives you the longest line in the particle in any direction. Once you identify your particle, you can pull out information like orientation, center X and Y, etc. Using the center position, orientation and length, you should be able to calculate the endpoints fairly accurately.
The main problem you could have is when fibers are touching or crossing each other. They will be treated as a single fiber, which probably isn't what you want. It would take much more advanced algorithms to fix that, though.
Bruce
Bruce Ammons
Ammons Engineering