total images(RGB) of 300. PAL - 25 frames/sec. I couldn't succeed and get Memory lock error and I could proceed more than 50 images using PCI 1411. I use imaq sequence in the vi. What is the relation between buffer size and the total number of images?It sounds like you already went into MAX and set the max number of buffers for IMAQ. This is an upper limit on the number of buffers you can allocate. From what I have been told, it is harmless to set this to a very large number. This controls the number of buffers, but does not affect the size of each buffer.
From what you describe, I would guess you are running out of memory in the computer. Make sure you have plenty of memory available to store all these images. To capture 300 frames at 1.3 Mb each, you will need a minimum of 512 Mb. It would be better to have 1024 Mb, so you have plenty of room for the operating system, software, and manipulating the images.
Bruce
Bruce Ammons
Ammons Engineering