Overlay midpoint

[Nghtcrwlr]

I am having an image that goes through several stages. At one stage an overlay is created on the image. But this image with overlay is not saved anywhere. My question now is: Is there a way to find the coordinates of the centroid (x) in the image with overlay ?Since I am not saving the file i cannot use IMAQ Read image & vision info vi.( correct me if i am wrong)
Any better ideas to deal with it?

 

[MoviJOHN]

To save an overlay with the image, you need to convert the image to RGB (copy, then convert), copy the overlay from the original, and then merge the overlay.  The overlay becomes destructive at this point, so any pixel data under the verlay lines is lost.