Hi S,
This is a known issue in VBAI 2.5. It looks like there was an underlying problem with how the image dimensions were being read in VBAI. A 640x480 image was not necessarily being interpreted that way in the software. So when the code is ported to LabVIEW, the dimensions for the ROIs are based on the incorrect image size. For the image in LabVIEW, the correct image dimensions are used, so the ROIs are shifted.
The end result is that VBAI 2.6 addresses that issue and repairs the problem. .
Seamus