kdalton,
I tried! Wow did I try. Unfortunately, even if you do a calibration and set up a coordinate system the custom overlay that is created during the display image step is purely based on the pixels. For example, if you create a custom overlay of a line that starts at coordinates 0,0 it will always start in the very top left corner of the image every time, not matter what calibration or coordinate system you have set up.
Nicholas C
National Instruments
Applications Engineering