I think Ben is right. Windows will limit a file up to a certain size, and I believe 4 GB is quite on the limit, I think Windows 95 was up to 2 GB, I think. But your solution is to split the file in multiple files. I'm very positive this is the hint for this issue. Since most of the OSs will limit the size, but in our side (NI) for example LabVIEW can represent image sizes in 32 bit format so is up to 2GB. It will depend on the system you are using and the data types, however I'm confident it might be the OS unless your OS allows files larger than 4GB.
Good luck!
Nestor S.
IMAQ/Motion Support
National Instruments
Nestor