Real-Time Measurement and Control

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use result of first picture as a variable in measurements of following pictures

How can i use a result/measurement of the first picture as a variable in the measurements of the following pictures of an inspection in vision builder AI?
 
I want to measure the extension of an assay with NI Vision Builder. How can I measure the assay in the first picture and substract its length from the following pictures, when it starts to stretch? My problem is not the substracting, but the reaccess of the length in picture 1.
 
Help?
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