Want to Build Electroporation Device

[brightfield]

An electroporator is used to pulse cells/tissues with current, which enables DNA to transport across cell membranes - enabling exogenous protein expression. Basically, the electroporator performs two functions: 1) Apply variable square-wave pulse: a typical example for my purposes would be 5V amplitude, 40 ms t_on, 900 ms t_off, N=4 pulses, ~100 mA 2) Measure current sourced Commercially, the electroporator we use costs between $13-15,000. I have never used LabView but it seems to me that this would be a perfect time to start. Basically, I need a function generator that can source enough current, and an acquisition card for monitoring current. So, can anyone here recommend me some part numbers and LabView software that I can use to make a "homemade" electroporator? I would greatly appreciate the advice/suggestions. Thanks.

[hoffiz]

Hi,

Let see we can skipped the software part of this application for know and concentrate on the hardware. First let’s try to find the function generator that will accomplish your specifications. Since you want to source 100 mA this narrows our products to the Power Supplies, NI PXI-4130 Power SMU.  If you don’t want to use any external circuitry this board might do the job.

I strongly suggest taking a look at this article: What Is a Source Measure Unit (SMU)? And let me know what you think.

Waiting for your reply

[brightfield]

Thanks, Jaime. It just so happens after I posted the message yesterday I found the PXI-4130. It seems to me like it can generate enough voltage and source current, but how would I input a finite number (let's say 4) of square wave pulses? Can that be done using the base LabView software package? Also, would I be able to measure current (or perhaps, resistance) simultaneously using the same electrodes?

[hoffiz]

Hi,

As I was researching to answer your question I found another post from you handling you issue. Electroporator.

I hope this was sufficient to answer your questions.