Hi,
Depending on the developing environment that you are using all you need to do is open the wav file, get the data and then you can use an example for analog output to generate the response.
To acquire the data you can also use the examples provided, there is one desicion to make: read time domain data or frquency domain data from the board. Depending on what you need.
Make sure that you have the NI-DSA driver installed if you want to read time domain data.
If you are doing this in LabVIEW there are VIs to read the wav file. If you are using CVI I can give you a piece of code to read and interpreter the wav file.
Hope this helps!
Juan Carlos
N.I.