The first VI 'Create Virtual Cannel' has an input called physical channel. This needs an input. Right click on the terminal and select Create>Constant. Then you can select the channel (something like Dev1/ai0).
To your 2nd Q: The assistant is a fast way to configure your measurement. You can later also convert it to the VIs you use now. Using the VIs gives you full programmatically control of the task. (The meaningful phrases are 'configure' and 'full programmatically').
Felix