Hi Veena,
first you have to use the threshold function to get a binary image, this shouldn't be difficult since you have white spots on black surface.
If the result image has only your spots well outlined, you can directly use the IMAQ Find Circles function to get informations of all the circles contained in your image. This returns an array of clusters of circles dimensions, radius and area.
If you want to select only some spots to be detected you have, after thresholding, add ROIs to your image and apply the IMAQ Find Circles only to the extracted image from ROI.
Keep in mind that the IMAQ Find Circles lets you set the min and max radius of the circles to be detected, use this parameters to exclude small disturbing parts on your image; second, this function wor
ks correctly only for circles that have a radius less than or equal to 256 pixels.
Good luck,
Alberto