Hi Corey!
Great answers, DF. DF is right about the PCI-6143 being a Simultaneously Sampling board. This actually means that there is an ADC converter for each of your AI channels. This guarantees the synchronization of your samples on the DAQ board. What DF was explaining about cable length, etc. is making sure the signals that you are measuring actually get to the DAQ board at the same time. And also like DF said, this should only be a problem if you are trying to measure frequencies higher than what is possible to measure with the DAQ board anyway.
On another note, when using an E-series or M-series board (with only one ADC converter), the sampling of the channels will still be "aligned" (off by microseconds). Only if you are doing extremely precise measurements would you need to be concerned with this issue. This is cost/accuracy tradeoff that most people are willing to make.
Hope this helps!
Travis H.
National Instruments
Travis H.
LabVIEW R&D
National Instruments