Try the following steps.
1. You need to generate a callback for the OK button. In the UI editor, right-click on your OK button, then select Generate Control Callback.
2. In the OK callback, under the EVENT_COMMIT case, read the value of "amplitude of positive" using GetCtrlVal(), then do your calculation and write the new value to "amplitude of negative" using SetCtrlVal(). To use the function panels of these functions, type GetCtrlVal (in your OK callback), then press Ctrl-P. Right-click on any parameter to get some help.
3. If "amplitude of negative" will always be updated by the program and not by the user, change the control to an Indicator. In the UI editor, double-click on the "amplitude of negative" control, and change the Control Mode from Hot to Indicator.
It sounds like you would benefit from working through the tutorial and looking at some of the sample projects the ship with CVI. From any CVI window, goto Help >> Contents >> Contents and browse the topics under Using LabWindows/CVI.
Welcome to CVI!