Machine Vision

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I am a new user of vision assistant and of treatment image.

With a microscop I acquire a image of nanoparticules.
And now I want to treatment this image with labview. 
The goal of this treatment and to know the distribution of size.
The particules are a ellipsoide form, so I must know the long side and
the short side. When I know the size of the two side, I calculate distribution of
size and the ratio.

I begin to work with vision assistant and now I work with labview.

I have some problems.
The first is :
The programm analyse only a part of image. The part is show in image out in front panel, the rest
of image doesn't analyse. Why?

the second is : 
I use the fonction Particle analysis for to now the 
equivalent rect long side and the equivalent rect short side.
Until now I work in pixel but I want to work in real world.
The particule measurements doesn't work but I don't know whty

Do you have any suggestion?

Thanks
 
PS : I know, that the particules are stacked. At the end of the programm I put a max limit and min limit
to eliminate the stackparticules and the scrap.
 
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Message 1 of 7
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First please attach a jpg image and dont rename a bmp image as jpg. The image is still trying to load here. Since the overlapped/stacked particles can be ignored, You can consider the area of the particle for this. Using a realworld measurement requires you to calibrate the dimension measurement. One simple way you can try is to manually calculate the number of pixels with mm.

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Message 2 of 7
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The problem with the jpg is the microscop give a tif format image. And I don't can to download a tif image in this forum. 

 

Sorry I don't think it can't make problem if the image is in jpg instead in tif. 

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Any update?
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Message 4 of 7
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Yes, I have some improvements.

But I don't know to calibrate my image.

 

Do you know to calibrate an image ?

I try with the fonction "IMAQ select Point" two times and then 

with the fonction "IMAQ Point Distances"? but I don't know

if it exist a best solution?

 

And I have an another problem.

After the fonction "particule analysis" I have a 2-D array, and after

I want a txt file with the measurements. For a 1-D array I know to convert into string

but for a 2-D array I have some difficulties.

 

Tanks for your help

 

Aurélie

 

My image

http://www.filedropper.com/v2271

 

 

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Message 5 of 7
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Use a reshape array and get the required elements out of a 2D array.
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