The simple answer is to put the 2 channels of interest in the Channel Control of your VI. Separated them with a comma.
To be honest your question is not very specific. There must be hundereds or thousands of ways to simultaneously acquire 2 analog inputs.
Try looking at the example VIs that ship with the software. Use the Find Examples function of the startup page for LV. Familiarize yourself with the information there and try some likely ones out. At this point you may have some more specific questions to ask.
The descriptions of the VI's are sometimes hard to read, but are usually enough to operate the VI.
Mike