Hey ianhf - I think there's a way to do what you want, but with a slightly different method than routing a data line to the PXI backplane. Data lines can only be driven out of the DDC Connector on the front panel of the device - they cannot be routed to the backplane. However, exported events can be, and they have the added benefit of not using up one of your data lines. There are two events that might get you what you need. The first is DataActive - it's a level event that is asserted whenever you're generation session is running. Thus it toggles at the start of the generation. This could be routed to the PXI backplane to trigger a start condition on another device.
Another type of event is a marker event. You can configure and control markers by using scripting on the 654x. Scripting allows HW timed linking/looping of multiple waveforms, but also allows you to generate a pulse whenever a certain point in a waveform is reached. You decide where in the waveform you want the marker to occur. A marker could be setup to pulse at multiple arbitrary positions within a waveform. Check out the following example that shows how to export a marker event
.../cvi/samples/nihsdio/dynamic generation with script/DynamicGenerationWithMarker/DynamicGenerationWithMarker.prj
This example routes the marker event to PFI0, but by changing the markerDestination to NIHSDIO_VAL_PXI_TRIG0_STR (a.k.a. "PXI_Trig0") you could route it to PXI Trigger Line 0 on the backplane. There's also pretty thorough documentation on the scripting language in the help file.
I hope this helps,
Chris